ra fls  (Cell Applications Inc)

Journal: Experimental & molecular medicine

Article Title: The HRAS-binding C2 domain of PLCη2 suppresses tumor-like synoviocytes and experimental arthritis in rheumatoid arthritis.

doi: 10.1038/s12276-025-01393-5

Figure Lengend Snippet: Fig. 1 Screening process and selection of the PLCη2_C2 domain, which affects the proliferation and NF-κB activity of RA FLSs. a, b Cell proliferation rates (a) and relative NF-κB activities (b) were measured in RA FLSs transduced separately with 144 different Ad-C2 vectors or the Ad- GFP vector for 48 h. Heatmaps display differences in the cell proliferation rate (a, left) and relative NF-κB activity (b, left). The heatmap areas show that Ad-C2 significantly altered the cell proliferation rate (a, right) and relative NF-κB activity (b, right). Red and green shading represents increased or decreased cell proliferation rates and NF-κB activities in the transduced RA FLS, respectively, compared with the corresponding values in the control transductants (Ad-GFP). c Top 15 Ad-C2 vectors in terms of altered cell proliferation rates (left) and relative NF-κB activities (right). d Venn diagrams showing the numbers of Ad-C2 vectors associated with significantly altered cell proliferation rates and relative NF-κB activities. e The overlap between Ad-C2 vectors associated with altered cell proliferation rates and altered NF-κB activities. The data shown represent vectors that led to reduced cell proliferation rates and NF-κB activities in RA FLSs at the same time compared with those obtained with Ad-GFP. Only Ad-C2 vectors with an enrichment score (−log(P value)) above 1.3 (P < 0.05) were considered for the analysis. *P < 0.05, **P < 0.01, versus the vector control (Ad-GFP). f, g Cell proliferation rates (f) and viabilities (g) of RA FLSs transduced with Ad-GFP, Ad-PLCη2_C2, Ad-SYTL-C2 or Ad-SYT4_C2 for 48 h. The data shown are expressed relative to vector control (Ad-GFP) values and represent the mean ± s.e.m. of three independent experiments involving three different RA patients. *P < 0.05, **P < 0.01, ***P < 0.001 versus control (untreated); ###P < 0.001 versus vector control (Ad-GFP), as determined by unpaired Student’s t-test (e) and one-way ANOVA followed by unpaired, two-tailed t-test (f and g).

Article Snippet: RA FLS proliferation was assessed using Cell Counting Kit-8 (CCK-8) assays (Dojindo, CK04).

Techniques: Selection, Activity Assay, Plasmid Preparation, Control, Transduction, Two Tailed Test

Journal: ACS Omega

Article Title: ROS-Scavenging Bamboo-Derived Carbon Dot-Methotrexate Nanocomposite Ameliorates Rheumatoid Arthritis through Dual Therapeutic Mechanisms

doi: 10.1021/acsomega.5c09772

Figure Lengend Snippet: Effects of the MTX/CD complex on RA-FLS viability and proliferation. (A) Cell viability of RA-FLS treated with various concentrations of CDs, as determined by the CCK-8 assay. (B) Representative images of colony formation in RA-FLS under different treatments. (C) Quantification of colony formation rates. (D) Transwell migration assay images. (E) Quantification of migrated cells per field. (F) Scratch wound healing assay images at 0, 24, and 48 h. (G) Quantification of the wound closure rate.

Article Snippet: RA-FLS were obtained from Wuhan Procell Life Technology Co., Ltd. (Cat. CP-H248).

Techniques: CCK-8 Assay, Transwell Migration Assay, Wound Healing Assay

Journal: ACS Omega

Article Title: ROS-Scavenging Bamboo-Derived Carbon Dot-Methotrexate Nanocomposite Ameliorates Rheumatoid Arthritis through Dual Therapeutic Mechanisms

doi: 10.1021/acsomega.5c09772

Figure Lengend Snippet: ROS scavenging and anti-inflammatory effects of the MTX/CD complex in RA-FLS. (A) Representative fluorescence images of intracellular ROS levels detected by DCFH-DA staining. (B) Quantitative analysis of ROS fluorescence intensity. (C) Real-time kinetics of superoxide generation measured by DHE fluorescence. (D) Calculated rates of ROS generation. (E–G) Serum levels of inflammatory cytokines (TNF-α, IL-6, IL-1β) measured by ELISA.

Article Snippet: RA-FLS were obtained from Wuhan Procell Life Technology Co., Ltd. (Cat. CP-H248).

Techniques: Fluorescence, Staining, Enzyme-linked Immunosorbent Assay

Journal: ACS Omega

Article Title: ROS-Scavenging Bamboo-Derived Carbon Dot-Methotrexate Nanocomposite Ameliorates Rheumatoid Arthritis through Dual Therapeutic Mechanisms

doi: 10.1021/acsomega.5c09772

Figure Lengend Snippet: Mechanisms of oxidative stress regulation and NF-κB pathway inhibition. (A) SOD activity in RA-FLS under different treatments. (B) CAT activity in RA-FLS under different treatments. (C) Western blot analysis of key proteins in the NF-κB pathway. (D–H) Densitometric quantification of protein expression levels: p-IKKβ, IKKβ, p-IκBα, IκBα, and COX-2, normalized to β-actin.

Article Snippet: RA-FLS were obtained from Wuhan Procell Life Technology Co., Ltd. (Cat. CP-H248).

Techniques: Inhibition, Activity Assay, Western Blot, Expressing